Analysis of Multiclass Pesticide Residues in Tobacco by Gas Chromatography Quadrupole Time-of-Flight Mass Spectrometry Combined with Mini Solid-Phase Extraction

A screening method using gas chromatography quadrupole time-of-flight mass spectrometry (GC-QTOF/MS) combined with mini solid-phase extraction (mini-SPE) was established for the quantification and validation of multiclass pesticide residues in tobacco. The quicker easier, sample purity higher than that obtained by traditional SPE dispersed-SPE. Box-Behnken design, an experimental design response-surface methodology, used to optimize variables affecting target recovery. Under optimized conditions, 92% pesticides showed satisfactory recoveries 70%–120% precision <20% at spiking levels 50, 250, 500 ng/g. limits detection all analyses were 0.05–29.9 ng/g 0.20–98.8 ng/g, respectively. In addition, a based on retention time homebuilt high-resolution database established. proposed parameters 100, 76.6%, 94.7%, 99.0% detected, respectively, workflow software. validated successfully applied analysis real tobacco samples. Thus, combination mini-SPE GC-QTOF/MS serves as suitable quantitative rapid